Composite

Part:BBa_K5269020:Design

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)


Golden Gate Assembly Site(tac promoter, BsmB I insert, L3S2P21)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
    Illegal BamHI site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Wenyuan Zhang, Hongrui Wu, Bryan Wei. Golden Gate Assembly of DNA Nanostructures. ACS Materials Letters. 2023 5(12), P.3316-3320 (https://pubs.acs.org/doi/10.1021/acsmaterialslett.3c01113#:~:text=Golden%20Gate%20assembly%20is%20a%20popular%20molecular%20cloning,system%20of%20synthetic%20DNA%20nanostructures%20in%20this%20work.)

References